|
|
|
|
|
| Wang,Tao; Jiang,An -li; Zhang,Jian-ye; Jing,Fei. |
| To observe effect of E2F decoy DNA on proliferation and apoptosis of androgen-independent prostate cancer cell line PC-3M the binding specificity of the E2F decoy DNA to the PC-3M nuclear protein was detected by electrophoretic mobility shift assay (EMSA). E2F decoy DNA, ARE decoy DNA and Control decoy DNA were respectively transfected into PC-3M cells with lipofectamine.Their influence on cell proliferative activity was detected by MTT assay.The cell apoptotic rate was determined by flow cytometric(FCM) analysis and chromosome DNA ladder was detected by DNA gel electrophoresis.The change of mRNA expression of C-myc and CyclinD1 were detected by RT-PCR.The change of mRNA expression of C-myc and CyclinD1 were detected by Western-blot. EMSA demonstrated... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Decoy DNA; Transcription factors; PC-3M cells; Apoptosis. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200011 |
| |
|
|
| Yang,Zhao; Zhuan,Bing; Yan,Ying; Jiang,Simin; Wang,Tao. |
| BACKGROUND: Although numerous efforts have been made, the pathogenesis underlying lung squamous-cell carcinoma (SCC) remains unclear. This study aimed to identify the CNV-driven genes by an integrated analysis of both the gene differential expression and copy number variation (CNV). RESULTS: A higher burden of the CNVs was found in 10-50 kb length. The 16 CNV-driven genes mainly located in chr 1 and chr 3 were enriched in immune response [e.g. complement factor H (CFH) and Fc fragment of IgG, low affinity Ilia, receptor (FCGR3A)], starch and sucrose metabolism [e.g. amylase alpha 2A (AMY2A)]. Furthermore, 38 TFs were screened for the 9 CNV-driven genes and then the regulatory network was constructed, in which the... |
| Tipo: Journal article |
Palavras-chave: Lung squamous-cell carcinoma; Differentially expressed genes; Copy number variation; Copy number variation-driven genes. |
| Ano: 2015 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100047 |
| |
|
|
| Guo,Huan; Zeng,Xiangting; Li,Haining; Guo,Yanxiang; Wang,Tao; Guo,Hongyun; Zhu,Gongjian; Wang,Lan; Zhou,Haihong; Liu,Kedan; Chen,Xiaoyi; Wang,Haitao; Zhao,Xinke; Su,Haixiang; Li,Yingdong. |
| Abstract Circulating miRNAs presenting in plasma in a stable manner have been demonstrated their potential role as a promising biomarkers in many human diseases, such as Alzheimer’s disease, melanoma and ovarian carcinoma. However, few circulating miRNAs could be used for breast ductal cancer diagnosis. Here, we identified miR-1273g-3p as a biomarker for detecting breast ductal cancer. We detected miR-1273g-3p levels in the plasma of 39 sporadic breast ductal cancer patients and 40 healthy donors by Stem-loop Quantitative Real-time PCR (qRT-PCR). The results showed the plasma miR-1273g-3p level were significantly up-regulated in breast ductal cancer patients compared with healthy donors (p=0.0139). Receiver operating characteristic (ROC) curve also... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Breast ductal carcinoma; Plasma; MiR-1273g-3p; Circulating miRNA; Biomarker. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652020000100801 |
| |
|
|
|
